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OriGene msrb2 orf clones

Cardiac complications occur in <t>MsrB2</t> KO DM. A . Glucose tolerance test (GTT) in WT and MsrB2 KO under nonDM and DM conditions. B . Body weight in WT and MsrB2 KO DM mice during HFD feeding. The body weight was weighed every week. C . Electron microscopy (EM) images in WT and MsrB2 KO DM mice. The white arrow indicates the mitochondria (autophagosome and autolysosome). The black triangle indicates the membrane structure. A white triangle indicates an abnormal Z-line. D . H&E and trichrome staining of histological sections of the heart of WT and MsrB2 KO mice under nonDM and DM MsrB2 conditions. E . Fibrotic areas were quantified using the ImageJ (FIJI) analyzer on histological sections. The two-way ANOVA analysis was performed for p values

Msrb2 Orf Clones, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/msrb2+orf+clones/Atp6ap1+(BC048241)+Mouse+Tagged+ORF+Clone/pmc11225187-33-4-8
Average 96 stars, based on 277 article reviews

msrb2 orf clones - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Methionine sulfoxide reductase B2 protects against cardiac complications in diabetes mellitus"

Article Title: Methionine sulfoxide reductase B2 protects against cardiac complications in diabetes mellitus

Journal: Diabetology & Metabolic Syndrome

doi: 10.1186/s13098-024-01390-0

Cardiac complications occur in MsrB2 KO DM. A . Glucose tolerance test (GTT) in WT and MsrB2 KO under nonDM and DM conditions. B . Body weight in WT and MsrB2 KO DM mice during HFD feeding. The body weight was weighed every week. C . Electron microscopy (EM) images in WT and MsrB2 KO DM mice. The white arrow indicates the mitochondria (autophagosome and autolysosome). The black triangle indicates the membrane structure. A white triangle indicates an abnormal Z-line. D . H&E and trichrome staining of histological sections of the heart of WT and MsrB2 KO mice under nonDM and DM MsrB2 conditions. E . Fibrotic areas were quantified using the ImageJ (FIJI) analyzer on histological sections. The two-way ANOVA analysis was performed for p values

Figure Legend Snippet: Cardiac complications occur in MsrB2 KO DM. A . Glucose tolerance test (GTT) in WT and MsrB2 KO under nonDM and DM conditions. B . Body weight in WT and MsrB2 KO DM mice during HFD feeding. The body weight was weighed every week. C . Electron microscopy (EM) images in WT and MsrB2 KO DM mice. The white arrow indicates the mitochondria (autophagosome and autolysosome). The black triangle indicates the membrane structure. A white triangle indicates an abnormal Z-line. D . H&E and trichrome staining of histological sections of the heart of WT and MsrB2 KO mice under nonDM and DM MsrB2 conditions. E . Fibrotic areas were quantified using the ImageJ (FIJI) analyzer on histological sections. The two-way ANOVA analysis was performed for p values

Techniques Used: Electron Microscopy, Membrane, Staining

Decreased autophagy and SERCA2a-PLN function in MsrB2 KO DM mice. A . A Western blot analysis of MsrB2, LC3I/II, Parkin, SERCA2a, pPNL, PNL, and GAPDH of nonDM (WT #1–3, MsrB2 KO #1–3) and DM mouse (WT #1–3, MsrB2 KO #1–6) hearts. GAPDH served as a loading control. B – D . Quantification of MsrB2, LC3I/II, Parkin, SERCA2a, pPNL/PNL, and GAPDH signal intensity. E . Quantitative RT-PCR analysis MsrB2 and SERCA2a transcript levels in nonDM (WT #1–5, MsrB2 KO #1–5) and DM (WT #1–3, MsrB2 KO #1–6) mouse hearts. The two-way ANOVA analysis was performed for p values

Figure Legend Snippet: Decreased autophagy and SERCA2a-PLN function in MsrB2 KO DM mice. A . A Western blot analysis of MsrB2, LC3I/II, Parkin, SERCA2a, pPNL, PNL, and GAPDH of nonDM (WT #1–3, MsrB2 KO #1–3) and DM mouse (WT #1–3, MsrB2 KO #1–6) hearts. GAPDH served as a loading control. B – D . Quantification of MsrB2, LC3I/II, Parkin, SERCA2a, pPNL/PNL, and GAPDH signal intensity. E . Quantitative RT-PCR analysis MsrB2 and SERCA2a transcript levels in nonDM (WT #1–5, MsrB2 KO #1–5) and DM (WT #1–3, MsrB2 KO #1–6) mouse hearts. The two-way ANOVA analysis was performed for p values

Techniques Used: Western Blot, Control, Quantitative RT-PCR

ROS induced methionine sulfoxidation (MetO) and mitochondrial dysfunction in the heart of diabetic mice. A . Blood Glucose in nonDM and DM mice. B . Western blot analysis of MetO and MsrB2 in nonDM (#1–3) and DM mouse hearts (DM #1–3). GAPDH served as a loading control. C . Quantification of MetO and MsrB2 signal intensity. D . Western blot analysis of Parkin and LC3II in nonDM (#1–3) and DM mouse hearts (DM #1–3). GAPDH served as a loading control. E . Quantification of Parkin and LC3II signal intensity. F . Western blot analysis of MsrB2 and LC3I/II in nonDM (#1–3), DM mouse hearts (DM #1–3), and DM with NAC treatment (#1–5). GAPDH served as a loading control. G . Quantification of MsrB2 and LC3II signal intensity. H . Electron microscopy (EM) in nonDM mice. The white body indicates a mitophagy structure. I . EM in DM mice. The White body indicates mitophagy (autophagosome and autolysosome) structure. J . Quantification of mitochondria size in nonDM and DM mice. K . Western blot analysis of DRP1 and DRP1 and Mfn2 in nonDM (#1–3) and DM mouse hearts (DM #1–3). GAPDH served as a loading control. L . Quantification of DRP1signal intensity. The nonparametric t -test was performed for p values

Figure Legend Snippet: ROS induced methionine sulfoxidation (MetO) and mitochondrial dysfunction in the heart of diabetic mice. A . Blood Glucose in nonDM and DM mice. B . Western blot analysis of MetO and MsrB2 in nonDM (#1–3) and DM mouse hearts (DM #1–3). GAPDH served as a loading control. C . Quantification of MetO and MsrB2 signal intensity. D . Western blot analysis of Parkin and LC3II in nonDM (#1–3) and DM mouse hearts (DM #1–3). GAPDH served as a loading control. E . Quantification of Parkin and LC3II signal intensity. F . Western blot analysis of MsrB2 and LC3I/II in nonDM (#1–3), DM mouse hearts (DM #1–3), and DM with NAC treatment (#1–5). GAPDH served as a loading control. G . Quantification of MsrB2 and LC3II signal intensity. H . Electron microscopy (EM) in nonDM mice. The white body indicates a mitophagy structure. I . EM in DM mice. The White body indicates mitophagy (autophagosome and autolysosome) structure. J . Quantification of mitochondria size in nonDM and DM mice. K . Western blot analysis of DRP1 and DRP1 and Mfn2 in nonDM (#1–3) and DM mouse hearts (DM #1–3). GAPDH served as a loading control. L . Quantification of DRP1signal intensity. The nonparametric t -test was performed for p values

Techniques Used: Western Blot, Control, Electron Microscopy

Electrophysiological dysfunctions occurred in MsrB2 KO DM mice. A ~ I . ECG recording results in nonDM (WT #1–4, MsrB2 KO #1–5) and DM (WT #1–11, MsrB2. KO #1–9) mouse hearts

Figure Legend Snippet: Electrophysiological dysfunctions occurred in MsrB2 KO DM mice. A ~ I . ECG recording results in nonDM (WT #1–4, MsrB2 KO #1–5) and DM (WT #1–11, MsrB2. KO #1–9) mouse hearts

Techniques Used:

MsrB2 induces autophagy by LC3 activation. A . Western blot analysis of MsrB2 and LC3I/II in H9C2 after MsrB2-GFP transfection. MsrB2 induced LC3II in a concentration-dependent manner. GAPDH served as a loading control. B . Quantification of MsrB2 and LC3II signal intensity. C . Western blot analysis of MsrB2, SERCA2a, and bMHC in WT and MsrB2 KO NMCM. After 4 h of starvation, 5.5 or 25 mM glucose was treated for 48 h. GAPDH served as a loading control. D . Quantification SERCA2a and bMHC signal intensity. E . Western blot analysis of MsrB2, LC3I/II, pDRP1, and DRP1 in NMCM after Ad-MsrB2-GFP infection. Ad-MsrB2-GFP 50 m.o.i infected into NMCM. Then, 5.5 or 25 mM glucose was treated for 48 h after 4 h starvation. GAPDH served as a loading control. F . Quantification of LC3II and pDRP1 signal intensity. The one-way ANOVA analysis was performed for p values

Figure Legend Snippet: MsrB2 induces autophagy by LC3 activation. A . Western blot analysis of MsrB2 and LC3I/II in H9C2 after MsrB2-GFP transfection. MsrB2 induced LC3II in a concentration-dependent manner. GAPDH served as a loading control. B . Quantification of MsrB2 and LC3II signal intensity. C . Western blot analysis of MsrB2, SERCA2a, and bMHC in WT and MsrB2 KO NMCM. After 4 h of starvation, 5.5 or 25 mM glucose was treated for 48 h. GAPDH served as a loading control. D . Quantification SERCA2a and bMHC signal intensity. E . Western blot analysis of MsrB2, LC3I/II, pDRP1, and DRP1 in NMCM after Ad-MsrB2-GFP infection. Ad-MsrB2-GFP 50 m.o.i infected into NMCM. Then, 5.5 or 25 mM glucose was treated for 48 h after 4 h starvation. GAPDH served as a loading control. F . Quantification of LC3II and pDRP1 signal intensity. The one-way ANOVA analysis was performed for p values

Techniques Used: Activation Assay, Western Blot, Transfection, Concentration Assay, Control, Infection

ROS induced methionine sulfoxidation (MetO) in human diabetic hearts. A . Western blot analysis of MetO in normal (NH #1–3) and diabetic human heart tissue(DH #1–6). GAPDH served as a loading control. B . Quantification of MetO signal intensity. C . Western blot analysis of methionine sulfoxide reductase A and B2 (MsrA and B2) in normal (NH #1–3) and diabetic heart tissue (DH #1–6). GAPDH served as loading a loading control. D . Quantification of MsrA and MsrB2 signal intensity. E . Western blot analysis of LC3l/II and p62 in normal (NH #1–3) and diabetic heart tissue (DH #1–6). GAPDH served as a loading control. F . Quantification of LC3l/II and p62 signal intensity. G . Western blot analysis of DRP1 and OPA1 in normal (NH #1–3) and diabetic heart tissues (DH #1–6). GAPDH served as a loading control. H . Quantification of MetO intensity

Figure Legend Snippet: ROS induced methionine sulfoxidation (MetO) in human diabetic hearts. A . Western blot analysis of MetO in normal (NH #1–3) and diabetic human heart tissue(DH #1–6). GAPDH served as a loading control. B . Quantification of MetO signal intensity. C . Western blot analysis of methionine sulfoxide reductase A and B2 (MsrA and B2) in normal (NH #1–3) and diabetic heart tissue (DH #1–6). GAPDH served as loading a loading control. D . Quantification of MsrA and MsrB2 signal intensity. E . Western blot analysis of LC3l/II and p62 in normal (NH #1–3) and diabetic heart tissue (DH #1–6). GAPDH served as a loading control. F . Quantification of LC3l/II and p62 signal intensity. G . Western blot analysis of DRP1 and OPA1 in normal (NH #1–3) and diabetic heart tissues (DH #1–6). GAPDH served as a loading control. H . Quantification of MetO intensity

Techniques Used: Western Blot, Control

Summary. Increased MsrB2 expression in diabetic mouse hearts suppressed cardiac complications. These results were confirmed by inducing diabetes in mice with suppressed MsrB2 expression (MsrB2 KO). In MsrB2 KO DM, the expression of SERCA2a, which regulates myocardial contractility, was reduced, cardiac fibrosis increased, and the number of mitochondrial structural abnormalities increased

Figure Legend Snippet: Summary. Increased MsrB2 expression in diabetic mouse hearts suppressed cardiac complications. These results were confirmed by inducing diabetes in mice with suppressed MsrB2 expression (MsrB2 KO). In MsrB2 KO DM, the expression of SERCA2a, which regulates myocardial contractility, was reduced, cardiac fibrosis increased, and the number of mitochondrial structural abnormalities increased

Techniques Used: Expressing

Clone Assay:

Article Title: Methionine sulfoxide reductase B2 protects against cardiac complications in diabetes mellitus
Article Snippet: .. We purchased Parkin and MsrB2 ORF clones from OriGene (USA) and subcloned them into RFP- and GFP-tagged vectors, respectively. .. Cherry-LC3 was purchased by Addgene (#40,827).

Article Title: Mitochondrial MsrB2 serves as a switch and transducer for mitophagy
Article Snippet: The isolated mitochondrial suspension was treated with or without cyclosporin A (CsA; Sigma, USA) at room temperature, followed by Western blot analysis. .. We purchased Parkin and MsrB2 ORF clones from OriGene (USA) and subcloned them into RFP‐ and GFP‐tagged vectors, respectively. .. The MsrB2‐GFP and pTagCFP‐Mito (Evrogen, Russia) were introduced into HEK293 or H9c2 with Lipofectamine 3000 (Invitrogen, USA) according to the manufacturer's protocol.

Article Title: Mitochondrial MsrB2 serves as a switch and transducer for mitophagy.
Article Snippet: The isolated mitochondrial suspension was treated with or without cyclosporin A (CsA; Sigma, USA) at room temperature, followed by Western blot analysis. .. We purchased Parkin and MsrB2 ORF clones from OriGene (USA) and subcloned them into RFP- and GFP-tagged vectors, respectively. .. The MsrB2-GFP and pTagCFP-Mito (Evrogen, Russia) were introduced into HEK293 or H9c2 with Lipofectamine 3000 (Invitrogen, USA) according to the manufacturer’s protocol.

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Journal: Diabetology & Metabolic Syndrome

Article Title: Methionine sulfoxide reductase B2 protects against cardiac complications in diabetes mellitus

doi: 10.1186/s13098-024-01390-0

Figure Lengend Snippet: Cardiac complications occur in MsrB2 KO DM. A . Glucose tolerance test (GTT) in WT and MsrB2 KO under nonDM and DM conditions. B . Body weight in WT and MsrB2 KO DM mice during HFD feeding. The body weight was weighed every week. C . Electron microscopy (EM) images in WT and MsrB2 KO DM mice. The white arrow indicates the mitochondria (autophagosome and autolysosome). The black triangle indicates the membrane structure. A white triangle indicates an abnormal Z-line. D . H&E and trichrome staining of histological sections of the heart of WT and MsrB2 KO mice under nonDM and DM MsrB2 conditions. E . Fibrotic areas were quantified using the ImageJ (FIJI) analyzer on histological sections. The two-way ANOVA analysis was performed for p values

Article Snippet: We purchased Parkin and MsrB2 ORF clones from OriGene (USA) and subcloned them into RFP- and GFP-tagged vectors, respectively.

Techniques: Electron Microscopy, Membrane, Staining

Journal: Diabetology & Metabolic Syndrome

Article Title: Methionine sulfoxide reductase B2 protects against cardiac complications in diabetes mellitus

doi: 10.1186/s13098-024-01390-0

Figure Lengend Snippet: Decreased autophagy and SERCA2a-PLN function in MsrB2 KO DM mice. A . A Western blot analysis of MsrB2, LC3I/II, Parkin, SERCA2a, pPNL, PNL, and GAPDH of nonDM (WT #1–3, MsrB2 KO #1–3) and DM mouse (WT #1–3, MsrB2 KO #1–6) hearts. GAPDH served as a loading control. B – D . Quantification of MsrB2, LC3I/II, Parkin, SERCA2a, pPNL/PNL, and GAPDH signal intensity. E . Quantitative RT-PCR analysis MsrB2 and SERCA2a transcript levels in nonDM (WT #1–5, MsrB2 KO #1–5) and DM (WT #1–3, MsrB2 KO #1–6) mouse hearts. The two-way ANOVA analysis was performed for p values

Article Snippet: We purchased Parkin and MsrB2 ORF clones from OriGene (USA) and subcloned them into RFP- and GFP-tagged vectors, respectively.

Techniques: Western Blot, Control, Quantitative RT-PCR

Journal: Diabetology & Metabolic Syndrome

Article Title: Methionine sulfoxide reductase B2 protects against cardiac complications in diabetes mellitus

doi: 10.1186/s13098-024-01390-0

Figure Lengend Snippet: ROS induced methionine sulfoxidation (MetO) and mitochondrial dysfunction in the heart of diabetic mice. A . Blood Glucose in nonDM and DM mice. B . Western blot analysis of MetO and MsrB2 in nonDM (#1–3) and DM mouse hearts (DM #1–3). GAPDH served as a loading control. C . Quantification of MetO and MsrB2 signal intensity. D . Western blot analysis of Parkin and LC3II in nonDM (#1–3) and DM mouse hearts (DM #1–3). GAPDH served as a loading control. E . Quantification of Parkin and LC3II signal intensity. F . Western blot analysis of MsrB2 and LC3I/II in nonDM (#1–3), DM mouse hearts (DM #1–3), and DM with NAC treatment (#1–5). GAPDH served as a loading control. G . Quantification of MsrB2 and LC3II signal intensity. H . Electron microscopy (EM) in nonDM mice. The white body indicates a mitophagy structure. I . EM in DM mice. The White body indicates mitophagy (autophagosome and autolysosome) structure. J . Quantification of mitochondria size in nonDM and DM mice. K . Western blot analysis of DRP1 and DRP1 and Mfn2 in nonDM (#1–3) and DM mouse hearts (DM #1–3). GAPDH served as a loading control. L . Quantification of DRP1signal intensity. The nonparametric t -test was performed for p values

Article Snippet: We purchased Parkin and MsrB2 ORF clones from OriGene (USA) and subcloned them into RFP- and GFP-tagged vectors, respectively.

Techniques: Western Blot, Control, Electron Microscopy

Journal: Diabetology & Metabolic Syndrome

Article Title: Methionine sulfoxide reductase B2 protects against cardiac complications in diabetes mellitus

doi: 10.1186/s13098-024-01390-0

Figure Lengend Snippet: Electrophysiological dysfunctions occurred in MsrB2 KO DM mice. A ~ I . ECG recording results in nonDM (WT #1–4, MsrB2 KO #1–5) and DM (WT #1–11, MsrB2. KO #1–9) mouse hearts

Article Snippet: We purchased Parkin and MsrB2 ORF clones from OriGene (USA) and subcloned them into RFP- and GFP-tagged vectors, respectively.

Techniques:

Journal: Diabetology & Metabolic Syndrome

Article Title: Methionine sulfoxide reductase B2 protects against cardiac complications in diabetes mellitus

doi: 10.1186/s13098-024-01390-0

Figure Lengend Snippet: MsrB2 induces autophagy by LC3 activation. A . Western blot analysis of MsrB2 and LC3I/II in H9C2 after MsrB2-GFP transfection. MsrB2 induced LC3II in a concentration-dependent manner. GAPDH served as a loading control. B . Quantification of MsrB2 and LC3II signal intensity. C . Western blot analysis of MsrB2, SERCA2a, and bMHC in WT and MsrB2 KO NMCM. After 4 h of starvation, 5.5 or 25 mM glucose was treated for 48 h. GAPDH served as a loading control. D . Quantification SERCA2a and bMHC signal intensity. E . Western blot analysis of MsrB2, LC3I/II, pDRP1, and DRP1 in NMCM after Ad-MsrB2-GFP infection. Ad-MsrB2-GFP 50 m.o.i infected into NMCM. Then, 5.5 or 25 mM glucose was treated for 48 h after 4 h starvation. GAPDH served as a loading control. F . Quantification of LC3II and pDRP1 signal intensity. The one-way ANOVA analysis was performed for p values

Article Snippet: We purchased Parkin and MsrB2 ORF clones from OriGene (USA) and subcloned them into RFP- and GFP-tagged vectors, respectively.

Techniques: Activation Assay, Western Blot, Transfection, Concentration Assay, Control, Infection

Journal: Diabetology & Metabolic Syndrome

Article Title: Methionine sulfoxide reductase B2 protects against cardiac complications in diabetes mellitus

doi: 10.1186/s13098-024-01390-0

Figure Lengend Snippet: ROS induced methionine sulfoxidation (MetO) in human diabetic hearts. A . Western blot analysis of MetO in normal (NH #1–3) and diabetic human heart tissue(DH #1–6). GAPDH served as a loading control. B . Quantification of MetO signal intensity. C . Western blot analysis of methionine sulfoxide reductase A and B2 (MsrA and B2) in normal (NH #1–3) and diabetic heart tissue (DH #1–6). GAPDH served as loading a loading control. D . Quantification of MsrA and MsrB2 signal intensity. E . Western blot analysis of LC3l/II and p62 in normal (NH #1–3) and diabetic heart tissue (DH #1–6). GAPDH served as a loading control. F . Quantification of LC3l/II and p62 signal intensity. G . Western blot analysis of DRP1 and OPA1 in normal (NH #1–3) and diabetic heart tissues (DH #1–6). GAPDH served as a loading control. H . Quantification of MetO intensity

Article Snippet: We purchased Parkin and MsrB2 ORF clones from OriGene (USA) and subcloned them into RFP- and GFP-tagged vectors, respectively.

Techniques: Western Blot, Control

Journal: Diabetology & Metabolic Syndrome

Article Title: Methionine sulfoxide reductase B2 protects against cardiac complications in diabetes mellitus

doi: 10.1186/s13098-024-01390-0

Figure Lengend Snippet: Summary. Increased MsrB2 expression in diabetic mouse hearts suppressed cardiac complications. These results were confirmed by inducing diabetes in mice with suppressed MsrB2 expression (MsrB2 KO). In MsrB2 KO DM, the expression of SERCA2a, which regulates myocardial contractility, was reduced, cardiac fibrosis increased, and the number of mitochondrial structural abnormalities increased

Article Snippet: We purchased Parkin and MsrB2 ORF clones from OriGene (USA) and subcloned them into RFP- and GFP-tagged vectors, respectively.

Techniques: Expressing

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msrb2 orf clones (OriGene)

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